Little Plants
Dec 18, 2009 Orchid Propagation
I checked on the flasks today. I could see little plants forming. They sure are tiny. Anyhow, it looks like the encyclia oncidioides, encyclia parviflora, brassavola cucullata and the brassavola nadosa all are starting to germinate. The only bad thing is that one of the brassavola cucullata flasks has some contamination. It looks like it started around the seeds, so the seeds probably weren’t disinfected enough (I will try a little more peroxide next time).
Little Orchid Plants
yucky mold
Tags: brassavola, encyclia, flasking, seed
Flasking – the process
Nov 15, 2009 Orchid Propagation
My flasking process. This only covers the actual process I used to sow my orchid seeds. Note that there are some preparation steps that you need to take before you are at this point (obtaining seed, obtaining sowing materials, preparing the flasks). Anyhow, I hope this helps you all out.
Prepare the seeds. The night before I sow the orchid seeds, I prepare my seeds. I separate a small quantity of seeds and put them into a small test tube. It is difficult to tell how many seeds, but I estimate a couple hundred or so. I then place a few drops (10-15) or water (distilled or Reverse Osmosis works fine) in the test tube. I let this sit over night.
Seed sowing day.
I set up my glove box with all my supplies and orchid seeds. The first thing I did was I put about 20 drops (more or less) of Hydrogen Peroxide into each test tube of orchid seeds. This is used to disinfect the seeds. The items I put in the glove box were: 8 mason jars (my flasks), my spray bottle of IPA (alcohol), 8 small glass pipettes (with the little rubber bulbs off of them), a test tube holder with the test tubes of seeds and a small bowl for used water/hydrogen peroxide.
I place my hands (no gloves) inside the box and spray down the inside with the IPA. I give each of my hands a spray also. I spray the pipets. Then I put the little rubber bulb on each of them. I let that sit for about 15 minutes while I take each test tube and swirl the seeds around in the peroxide.
Next, I take the rings off of the mason jars and spray the inside of the glove box with the IPA again. Oh, yeah, it is a good idea to do this in a well ventilated area (open the windows or something). I carefully remove the lids from 2 mason jars. I sow 2 flasks of each variety in case of contamination. I use a new pipette for each flask. I dip the pipette into the seeds/hydrogen peroxide and remove some excess (and put it in the bowl). If you keep the amount of water and peroxide low in the first place, you can skip that step. I fill the pipette with the seeds and peroxide. Then I drop about 10 drops into the flask. I have tried just making drops and then some where I swirled the mixture around in the flask a little bit. I will have to wait to see if either works better.
I close up the flask and tighten the rings. Then I move on to the next variety. If your aquarium is small, you can move the flasks out at that time and then just remember to re-spray a little IPA on your hands and your work area – this has worked fine for me.
For my first flasking, I used the following seeds:
encyclia alta (from the Orchid Seedbank Project – #3153)
encylia oncidioides (OSP #3695)
dendrobium secundum (ebay special)
dendrobium unicum (another ebay special)
Flasking – the setup
Nov 15, 2009 Orchid Propagation, orchids
My first try at flasking. What an adventure.
I read many articles on growing orchids from seeds. I felt a little overwhelmed. There are so many different techniques. I sort of took all the information in and came up with my own way of doing it – a combination of all I read.
My biggest concern was contamination. I read so much about how difficult it is to make sure everything is “as clean as possible”. Unless you have the resources to have a dedicated lab, it can be a little tricky. Of course, I wanted the lab with the laminar flow hood and all the bells and whistles. What I ended up with is much different. Since this is just for fun, it is hard to justify spending much money on growing orchids from seeds. Here is my “lab” or at least part of it.
my orchid glove box
As you can see my “lab” is nothing special. In fact, everything I used was just sitting around the house. I am using an aquarium which is way too small. I have a spray bottle of IPA for sterilizing the glove box and everything inside of it. Then I have my test tubes of orchid seeds. I have 8 flasks (mason jars), a little bowl for “used” water/hydrogen peroxide, and some pipets. The opening is closed using a couple of dish towels – I told you it wasn’t anything special. And, yes, it is set up on the kitchen counter.
I will post my process in the next post.
Orchid Flasking
Nov 1, 2009 Orchid Propagation
I received my orchid seed sowing media a couple days ago.
Today, I made some jars (8 of them). I am using 1/2 pint wide mouth jars. I cooked them in a pressure cooker (15 psi for 15 minutes) to sterilize them. This worked out ok, but they ended up being sealed (with a vacuum), so it might lead to problems when I sow the seeds. I will let these sit for about a week and see if there is any contamination. I tried to follow the instructions from here, but I didn’t have a good way to let the jars cool down since I was only cooking two at a time. I had to open the cooker and tighten the bands to form the seal. It is all a learning experience.
I think I will prepare another 8 jars using the method from Lotte & Thomas Orchids. I will try to do that sometime during the next week. They prepare the flasks in an oven at 300 degrees F for 30 minutes and then let them cool in the oven. This might be and easier solution.
I also thought that if I tried using a different type of jar (like a baby food jar or smaller canning jars), I might be able to cook more at a time and let them cool over night. I might experiment with that also.
Orchid Fasking
Oct 23, 2009 Orchid Propagation
Just an update on my orchid flasking (growing from seed) project.
I am still waiting on my seed sowing media. I ordered from Phytotechlab. I ordered some P723 (seed sowing media) and P785 (replate media). They seem to be a little slow (I think something is on back-order). Anyhow, I am not in any rush, so it is no big deal.
I have just about everything else ready, so when that comes in, I can get started.
